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The protein expression of PI3K, AKT, mTOR and their respective phosphorylated forms detected by Western blotting

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder

doi: 10.1007/s00018-026-06096-2

Figure Lengend Snippet: The protein expression of PI3K, AKT, mTOR and their respective phosphorylated forms detected by Western blotting

Article Snippet: For immunofluorescence staining, free-floating hypothalamic sections were co-incubated with primary antibodies against somatostatin (SST) (1:100, rabbit, bs-37040R, Bioss) and growth hormone-releasing hormone (GHRH) (1:100, rabbit, bs-0205R, Bioss), while prefrontal cortical sections were incubated with primary antibodies targeting growth hormone (GH) (1:1000, rabbit, GB113303-100, Servicebio), phospho-AKT (1:100, rabbit, bs-0876R, Bioss), phospho-mTOR (1:100, rabbit, bs-3495R, Bioss), growth hormone receptor (GHR) (1:100, rabbit, bs-0654R, Bioss), and somatostatin receptor 2 (SSTR2) (1:100, rabbit, bs-10986R, Bioss).

Techniques: Expressing, Western Blot

Immunofluorescence analysis of GHR and SSTR2 in the prefrontal cortex. A Proposed signaling pathway of GH in regulating the PI3K/Akt/mTOR axis (KEGG Entry: map04935). B Quantitative analysis of the expression levels of GHR and SSTR2. C Representative immunofluorescence images of GHR and SSTR2 (50.0×, Scale bar: 20 μm). The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder

doi: 10.1007/s00018-026-06096-2

Figure Lengend Snippet: Immunofluorescence analysis of GHR and SSTR2 in the prefrontal cortex. A Proposed signaling pathway of GH in regulating the PI3K/Akt/mTOR axis (KEGG Entry: map04935). B Quantitative analysis of the expression levels of GHR and SSTR2. C Representative immunofluorescence images of GHR and SSTR2 (50.0×, Scale bar: 20 μm). The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group

Article Snippet: For immunofluorescence staining, free-floating hypothalamic sections were co-incubated with primary antibodies against somatostatin (SST) (1:100, rabbit, bs-37040R, Bioss) and growth hormone-releasing hormone (GHRH) (1:100, rabbit, bs-0205R, Bioss), while prefrontal cortical sections were incubated with primary antibodies targeting growth hormone (GH) (1:1000, rabbit, GB113303-100, Servicebio), phospho-AKT (1:100, rabbit, bs-0876R, Bioss), phospho-mTOR (1:100, rabbit, bs-3495R, Bioss), growth hormone receptor (GHR) (1:100, rabbit, bs-0654R, Bioss), and somatostatin receptor 2 (SSTR2) (1:100, rabbit, bs-10986R, Bioss).

Techniques: Immunofluorescence, Expressing, Control

Immunofluorescence analysis of GH and p-AKT/GH and p-mTOR co-localization in the prefrontal cortex. A Representative immunofluorescence images of GH and p-Akt (50.0×, Scale bar: 20 μm). B Quantification of the Pearson’s correlation coefficient (R value) for GH and p-Akt co-localization. C Representative immunofluorescence images of GH and p-mTOR (50.0×, Scale bar: 20 μm). D Quantification of the Pearson’s correlation coefficient (R value) for GH and p-mTOR co-localization from. The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Microbiota-gut-brain axis and neuroendocrine pathways underlie divergent mechanisms of intermittent and continuous theta-burst stimulation in autism spectrum disorder

doi: 10.1007/s00018-026-06096-2

Figure Lengend Snippet: Immunofluorescence analysis of GH and p-AKT/GH and p-mTOR co-localization in the prefrontal cortex. A Representative immunofluorescence images of GH and p-Akt (50.0×, Scale bar: 20 μm). B Quantification of the Pearson’s correlation coefficient (R value) for GH and p-Akt co-localization. C Representative immunofluorescence images of GH and p-mTOR (50.0×, Scale bar: 20 μm). D Quantification of the Pearson’s correlation coefficient (R value) for GH and p-mTOR co-localization from. The data are presented as mean ± SD ( n = 3). ### p < 0.001 vs. control group; *** p < 0.001 vs. VPA group

Article Snippet: For immunofluorescence staining, free-floating hypothalamic sections were co-incubated with primary antibodies against somatostatin (SST) (1:100, rabbit, bs-37040R, Bioss) and growth hormone-releasing hormone (GHRH) (1:100, rabbit, bs-0205R, Bioss), while prefrontal cortical sections were incubated with primary antibodies targeting growth hormone (GH) (1:1000, rabbit, GB113303-100, Servicebio), phospho-AKT (1:100, rabbit, bs-0876R, Bioss), phospho-mTOR (1:100, rabbit, bs-3495R, Bioss), growth hormone receptor (GHR) (1:100, rabbit, bs-0654R, Bioss), and somatostatin receptor 2 (SSTR2) (1:100, rabbit, bs-10986R, Bioss).

Techniques: Immunofluorescence, Control